Comparison of Enteric-Tek with API 20E and conventional methods for identification of Enterobacteriaceae
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چکیده
منابع مشابه
Comparison of Enteric-Tek with API 20E and conventional methods for identification of Enterobacteriaceae.
Enteric-Tek (Flow Laboratories, Inc., Roslyn, N.Y.) is a new system designed for the identification of Enterobacteriaceae and consists of a round, multicompartmented plastic plate with 11 peripheral wells and 1 center well incorporating 14 biochemical reactions. Only one reagent (Kovacs reagent) is required to complete the biochemical test results for generation of a five-digit number for compu...
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The API 20E, the Enterotube, and routine methods of Cowan and Steel were used in parallel to identify 245 members of the Enterobacteriaceae. The API 20E and conventional methods gave the same identification in all but 2 (0-8%) of the 245 organisms tested. The Enterotube correctly identified 85% of these organisms on the first testing. On re-testing those organisms incorrectly identified the Ent...
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The Micro-ID system for rapid (4 h) identification of Enterobacteriaceae was evaluated by testing 433 enteric bacilli and 9 other gram-negative bacilli. Each isolate was identified with conventional tubed media and was also tested in the Micro-ID and API 20E systems. The overall accuracy of both systems was 97%. Micro-ID tests for the Voges-Proskauer reaction, indole and H2S production, and orn...
متن کاملThe comparison of PCR technique and API-20E kit with the conventional biomedical methods for the identification of Salmonella species in laboratory
Abstract Bachground and objectives: Salmonella is one of the most important agents of gastrointestinal infection and diarrhea in our country. Misdiagnosis of these bacteria leads to cure failure. The aim of this study was to make a comparison between PCR and the API-20E and conventional biochemical tests carried out for the identification of Salmonella. Material and Methods: In this study 470 s...
متن کاملTime-motion and cost comparison study of micro-ID, API 20E, and conventional biochemical testing in identification of Enterobacteriaceae.
A total of 730 Enterobacteriaceae strains isolated from 567 cultures were evaluated by a rapid kit method (Micro-ID; General Diagnostics, Morris Plains, N.J.; 4 h), an overnight incubation kit method (API 20E; Analytab Products, Plainview, N.Y.), and conventional biochemical test methodology (mostly overnight incubation and some rapid methods) to compare the amount of laboratory effort required...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1982
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.15.1.16-18.1982